The effect of isoflavonoid contents in SH003 and its subfractions on breast cancer

Objectives: We investigated the isoflavone contained in SH003 and its fractions, effect of these components on inhibition breast cancer.Methods: The isoflavones solvent fractions extract were identified by UPLC-MS contents quantified using HPLC analysis. estrogenic activity or was assessed ERE luciferase assay estrogen receptor (ER)-positive MCF-7 cells. To test cancer inhibitory effect, cell viability measured an MTT assay.Results: In this study, we demonstrated that contain 4 which are calycosin-7-β-D-glucoside, formononetin-7-β-D-glucoside, calycosin, formononetin. Despite containing isoflavones, estrogen-dependent transcription not altered both fractions. On other hand, inhibited cancer. addition, also showed reduced various cells.Conclusions: Overall, phytoestrogen included did influence activity, emphasizing safety treatment.